Further downstream in anterograde membrane traffic, endosomal maturation defects result in various functional and structural anomalies of the vacuole. We show here that bacterial proteins from Legionella pneumophila, when overexpressed, can inhibit multivesicular body formation, leading to missorting of the hydrolase Carboxypeptidase Y and to the formation of an aberrant membrane compartment adjacent to the vacuole. A genetic screen was used to identify such proteins, all of which were subsequently found to be released into the host cytosol upon Legionella infection.We wanted to use yeast in a new way as a gene discovery tool to identify previously unrecognized effectors that inhibit ... bacterial effectors normally involved in mammalian infection retain their biological function in yeast (Lesser and Miller, 2001; ... do they appear to inhibit organelle trafficking by the same mechanisms.
|Title||:||Vacuole Biogenesis, Function, and Size Control in Saccharomyces Cerevisiae: Central Roles for Membrane Trafficking and Lipid Signaling|
|Author||:||Jem A. Efe|
|Publisher||:||ProQuest - 2007|