Ubiquitin and Protein Degradation, Part B will cover chemical biology, ubiquitin derivatives and ubiquitin-like proteins, deubiquitinating enzymes, proteomics as well as techniques to monitor protein degradation. The chapters are highly methodological and focus on application of techniques. *Second part of the Ubiquitin and Protein Degration series *Topics include: E1 Enzymes, E2 Enzymes, E3 Enzymes, Proteasomes, and Isopeptidases169 limitations, 166 regeneration conditions, 173 troubleshooting, 172, 175 Tandem ubiquitin gene construction, 65-67 ... preparation, 245-246 expression plasmids, 244 nickel affinity chromatography purification of ubiquitinated proteins .
|Title||:||Ubiquitin and Protein Degradation|
|Author||:||Raymond J. Deshaies|
|Publisher||:||Gulf Professional Publishing - 2005|