For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the third of three on the topic of Translation Initiation includes articles written by leaders in the field.purification of FLAG-tagged complexes from yeast affinity purification, 9 buffers, 6 a8 cell growth and harvest, 8 cell lysis, 8a9 FLAG ... 2a3 eIF3 clustered alanine mutagenesis, 29a30 deletion/mutation analysis in yeast nickel affinity chromatography of subcomplexes, 25a27 ... affinity tagging, 21a22 characterization, 23a24 extract preparation, 22a23 nickel pull-down, 23 troubleshooting, 24 structure, anbsp;...
|Title||:||Translation Initiation: Cell Biology, High-throughput and Chemical-based Approaches|
|Publisher||:||Academic Press - 2007-10-12|