Even with secondary structure as a guide, decisions about alignment can be subjective. ... Since 1998, the journal Mycologia has required that authors of phylogenetic studies submit alignments and trees to TreeBASE before publication. ... The priming problem can sometimes be solved by using degenerate primers consisting of sequence mixtures that complement several different ... However, to date, relatively few fungal phylogenetic studies use mitochondrial protein-coding genes.
|Author||:||Karl Esser, Paul A. Lemke|