John R. Crowther provides today's bestselling practical guide to the understanding and application of ELISA. This important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, and statistical requirements for data examination. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology.This chapter examines the aspects of using ELISA to solve problems, definitions of terms met in serology, antibody structure and the production of antibodies in animals, units, dilutions, and molarities. AntibodiesaA Laboratory Manual (1) is ananbsp;...
|Title||:||The ELISA Guidebook|
|Author||:||John R. Crowther|
|Publisher||:||Springer Science & Business Media - 2000-01-01|