A Hybaid TR1 or Perkin- Elmer 2400 thermocycler was programmed with a 4 min hold at 94AdC; 30 cycles of 1 min at 94AdC, ... The Lb. xylosus xylA gene was sequenced manually according to the doubled-stranded DNA method of Wang ( 74).
|Title||:||Strain-specific detection and characterization of the xyl and xyn loci in Lactococcus lactis|
|Author||:||Karn A. Erlandson|