These cultures were mixed with equal volumes of sterile 20% glycerol (Sigma) and dispensed in 1.5 ml aliquots to ... Tne secondary or test culture was prepared w nh a iO^i inoculum from the primary culture and incubated for 20 to 24 h at 37a#39;C without shaking. ... with tap water, the same quat was included twice, once with tap water and once with deionized water as the diluents. A 3-liter batch of each solution was prepared according to the label instructions of each manufacturer.
|Title||:||Review of EPA's program to assure the safety and effectiveness of disinfectant products|
|Author||:||United States. Congress. House. Committee on Government Operations. Environment, Energy, and Natural Resources Subcommittee|
|Publisher||:||Government Printing Office - 1991|