Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological techniques that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.A ? u U A C A G U C A ? biotin raquot;AdAli avidin P digoxygenin O fluorophore Y antibody 3 Indirect Fluorescence Labeling ... As indicated in the diagram, the fluorophore is conjugated directly yribonucleotides (here, dUTP), which is incorporated into the probe DNA via e text). ... bound by fluorophore-labeled intisheep antibodies, thus aquot;amplifyingaquot; the fluorescent signal. low the cells to proceed through DNAanbsp;...
|Author||:||Jack H. Mydlo|
|Publisher||:||Springer Science & Business Media - 2001-01-01|