The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. The most useful features of this manual for your classroom and laboratory are: * Comb-bound, three-column, large 9-1/4q x 7-1/2q format * Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts * Necessary reagents and equipment are presented in a checklist at the beginning of each protocol * Techniques for bacteria are complemented with those for Drosophila * Each experiment has been tested in the laboratory by students for five years * Features a complete chapter on computers in the molecular biology laboratory * Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strainsE. coli strains I C600 [F * thi-I thr-I leuB6 lacY] tonAZl supE44 Aa#39;] HB101::Tn5 (Kma#39; ) [F-, hstZO (r81 mBa#39;) recA13 ara14 pr0A2 lacY] galK2 rpsLZO (Sma#39;) xyl-5 mil-I supE44 ATn5 kan (Kma#39;)] XLlaBlue ... (1987) and Soto-Gil and Zyskind (1989).
|Title||:||Recombinant DNA Laboratory Manual, Revised Edition|
|Author||:||Judith W. Zyskind, Sanford I. Bernstein|
|Publisher||:||Academic Press - 2012-12-02|