Gene duplication is one of the major forces driving genome evolution. Our study focuses on a particular retrogene Rcd-1 related (Rcd-1r) that originated through retroposition of the parental gene Required for cell differentiation 1 (Rcd-1) about 5-13 million years ago. Rcd-1r is present in D. melanogaster, D. simulans, D. sechellia and D. mauritiana whereas the parental gene is present in all the Drosophila species examined. Rcd-1r is inserted in the 3' UTR of the gene CG13102 and it is encoded in the complementary strand. Previous studies by Castrillion et al. showed that a P element inserted in the 5' UTR of Rcd-1r (that it is also the 3' UTR of CG13102) resulted in male sterility. The aim of this study was to find out which of the two genes CG13102 or Rcd-1r is responsible for the sterility phenotype associated with the P element insertion and to study Rcd-1r molecular evolution.A diagram of the results of the 5a#39; Race is depicted in figure 3.4. The PCR product obtained from 5a#39;RACE for fly halves of non curly males was then sequenced to confirm length of the transcriptional start site of Rcd-1r in mutant males.
|Title||:||Rcd-1 Related: A Positively Selected Retrogene with Spermatogenesis Function in Drosophila|
|Publisher||:||ProQuest - 2007|