This book is a complete guide to the technique of fluorescence microscopy. It describes the history, principles, and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. In addition, there is a discussion of the exciting developments in confocal fluorescence microscopy, which allows the three-dimensional distribution of particular substances to be determined.experience is that this often actually gives a lower reading than a blank field, since it may block light reflected from below. ... the spectrum will result in lower readings at the end of the spectrum measured last, and the spectrum may change as a result ... In my hands, this method has proved possible but time- consuming, and it is doubtful if the fading rate can be obtained ... the detection system should be as efficient as possible: a high-aperture monochromator or high-transmission filters, anbsp;...
|Title||:||Quantitative Fluorescence Microscopy|
|Author||:||F. W. D. Rost|
|Publisher||:||Cambridge University Press - 1991|