These two volumes present a comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.It is also common practice to use manual counting methods in the routine laboratory if the platelet count is low or there are atypical platelets present in the sample. The current international reference method for platelet counting is stillanbsp;...
|Title||:||Platelets and Megakaryocytes|
|Author||:||Jonathan M. Gibbins|
|Publisher||:||Springer Science & Business Media - 2004-01-01|