A cutting-edge collection of diverse readily reproducible techniques for dissecting the molecular mechanisms of pain transduction. These methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard and cutting-edge methods, but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.2.2.3. Constructing the cc-Sleeve 1. 1-cc plastic syringe. 2. One-sided razor blade . 3. Manual metal tube-cutter with a cutting diameter range starting from 1/8 in. 4. Hand drill (Multipro Dremel Drill with variable speed; Dremel, anbsp;...
|Author||:||Z. David Luo|
|Publisher||:||Springer Science & Business Media - 2004-01-01|