Neural stem cells offer a valuable model system for delineating the cellular and developmental processes in normal and diseased states of the central nervous system. In particular, neural stem cells have huge potential in regenerative medicine, owing to their expansion capability in culture and the ability to differentiate into multiple sub-neural lineages. Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. Including an overview of progress in the field over the past decade, Neural Stem Cell Assays is a one-stop reference for consistent methods and reliable tools that span the entire assay work flow, from isolation or generation of neural stem cells to characterization, manipulation and final application of neural stem cells in disease paradigms such as Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis. An excellent source of information for academic, pharmaceutical and biotechnology researchers who are new to the neural stem cell field, Neural Stem Cell Assays is an invaluable to experienced users who wish to integrate newly developed tools and technologies into their workflow. The book also covers important course material for students at the undergraduate and graduate level who are learning the basics of neural stem cell cultures, and differentiation to sub-neural lineages.Add 10I¼l of CellLight BacMam 2.0 reagent per 100, 000 cells to the growth media (see Note 12), and return to the ... Any research-grade fluorescence microscope equipped with suitable optics and digital acquisition can be used to collect ... camera (Photometrics) with pixel size of 91 nm. ... length and cell body area on a per-field basis in a manner that minimizes subjectivity and laborious manual tracing.
|Title||:||Neural Stem Cell Assays|
|Author||:||Navjot Kaur, Mohan C. Vemuri|
|Publisher||:||John Wiley & Sons - 2014-11-24|