A microfluidic device has been demonstrated that is capable of accurately monitoring bacteria levels in aqueous suspensions. The device uses nanoliter samples, thus providing very rapid, highly quantitative measurements. This method uses immunogenicity of bacteria to stain them with fluorescently labeled antibody specific to the target. Bacteria are then quantified by the fluorescence emission causing spikes in the output signal as they pass through the detection zone inside the microchip. This method has also been optimized to work where bacterial cells are not required to be washed after staining to make the process automated. The potential application of this device may be in continual monitoring of the harmful bacteria in coastal ocean waters that pose threat to the marine ecosystems.sample is necessary to ensure fast cooling rates required for vitrification as well as to provide enough transmission of ... biological samples such as cells and organelles, viruses and proteins, which confirm the successful application of this anbsp;...
|Title||:||Microstructure Studies in Synthetic and Bio-colloids, Imaging and Applications|
|Author||:||Ashish Kumar Jha|
|Publisher||:||ProQuest - 2008|