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This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.Manual system: This is the simplest system, and is very useful and relatively inexpensive. ... Automatic systems: The automatic systems allow the operator to microin- ject more than a thousand cells in a short period of time, but the system isanbsp;...

Author:Juan Carlos Lacal, Rosario Perona, James Feramisco
Publisher:Springer Science & Business Media - 1999-01-01


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