We have constructed E. coli strain devoid of gene encoding ptsG. The ptsG is involved in the transport of glucose and plays an important role in carbon catabolite repression. The ptsG-strain didn't show diauxic growth and consumed glucose and xylose simultaneously. It grew slower than wild type grown on glucose and had a long lag time. Our flux analysis revealed that slow growth of ptsG mutant is due less efficient transport of xylose and glucose in the mutant which results in less available ATP for biomass synthesis.10% U-13C xylose, 10% 1-13C xylose and 80% naturally labeled xylose was used as carbon substrate. Analytical procedures Dry cell weight was monitored by measuring optical density (OD550) using spectrophotometer (Genesys 20, anbsp;...
|Title||:||Metabolic Engineering of Escherichia Coli for the Efficient Utilization of Plant Sugar Mixture|
|Author||:||Madhuresh Kumar Choudhary|
|Publisher||:||ProQuest - 2008|