Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.Reagents required: a NCSU23 or PPMI [followed by PPM2 at 72 hpi (hours post insemination)] 1. The presumptive ... Make drops (25 lC10 ml oil +25 l) in 1007 dishes as well as three 250 l drops (125 lC oil C125 l) in a 1007 dish for washing. Place in the ... (If you are mounting embryos on a slide to check for fertilization, place the zygotes to be mounted in the wash drops back into the incubator. At 12 hpianbsp;...
|Title||:||Mammalian and Avian Transgenesis - New Approaches|
|Author||:||Shirley Pease, Carlos Lois|
|Publisher||:||Springer Science & Business Media - 2007-07-31|