The first edition of this manual appeared in 1992 and was entitled ECAT Assay Procedures. It was the result of a unique cooperation between experts brought together by the European Concerted Action on Thrombosis and Disabilities (ECAT). The Concerted Action was at that time under the auspices of the Commission of the European Union. The second edition, like the first edition, deals with diagnostic tests within the field of thrombosis. However, the second edition has a broader scope because it is no longer limited by the frontiers of ECAT. Experts allover the world, in and outside ECAT, have contributed to this edition. The editors are very grateful for their contributions. The need for a new edition is obvious. Since 1992 new assays have been introduced for research, diagnosis, and therapy of thrombosis; for other assays improvements have been suggested, while a few others became redundant. The editors waived the radioimmunoassays of ~-thrombog1obulin and platelet factor 4 due to the fact that the kits required for these assays are rarely, or no longer, available. Also the PAI-1 activity assay was waived as it is liable to many inconsistencies and to large variations. A list of names and addresses of manufacturers marketing the kits and reagents has been compiled, together with a list of the recommended nomenclature of quantities in thrombosis and haemostasis, in order to facilitate the use of the updated version. These lists have been carefully compiled by Johannes J. Sidelmann, PhD, Department of Clinical Biochemistry in Esbjerg, Denmark.Procedure This applies to the manual (tilt-tube) technique used in combination with the above reagents. ... Pipette 0.1 ml CaCl2 buffer and simultaneously start a stopwatch. Mix and tilt ... Repeat the measurement for the same plasma with APC /CaCl2 buffer instead of CaCl2 buffer. The above procedure can also be carried out on plasma prediluted 1:5 in FV-deficient plasma (STA Factor V, Diagnostica Stago). ... Many laboratories use a commercial APTT-based method ( Chromogenix).
|Title||:||Laboratory Techniques in Thrombosis - A Manual|
|Author||:||J. Jespersen, R.M. Bertina|
|Publisher||:||Springer Science & Business Media - 2000-06-30|