In Vitro Fertilization and Embryo Transfer

In Vitro Fertilization and Embryo Transfer

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The use of human in vitro fertilization in the management of infertility is the outgrowth of years of laboratory observations on in vitro sperm-egg interaction. qThe editors of this work have themselves contributed significantly to basic knowledge of the mammalian fertilization process. The observations of Don Wolf on sperm penetration, the block to polyspermy and, most recently, sperm hyperactivation in the monkey and human, Gregory Kopf's elucidation of the mechanisms of sperm activation during penetration and the reciprocal dialogue between sperm and egg, and Barry Bavister's definition of culture conditions and requirements necessary for in vitro oocyte maturation, fertilization and development in model mammalian systems including nonhuman primates have contributed greatly to our understanding of the mammalian fertilization process. Wolf, Kopf and Gerrity have enjoyed substantial interaction with clinicians in Departments of Obstetrics and Gynecology and have been directly involved with successful IVF programs. Both Wolf and Kopf have served as research scientists in the Division of Reproductive Biology at the University of Pennsylvania, which, for more than 22 years, has fostered co-mingling of clinically oriented and basic science faculty. It is through such interaction, which clearly exists at many institutions including the University of Wisconsin, that the process of technology transfer is best served. Without an exquisitely coordinated laboratory, there can be no consistent success in human in vitro fertilization. Quality control is pivotal, but close collaboration between the laboratory and the clinic is also essential as information is shared and correlated.A Manual of Basic Techniques Don P. Wolf. Fig. ... D6 Dense D7 onal Armount De LORONA Radiatin, D1 Denses compact D2 Clumpina#39; D5 absent D4 B-led D5 cat Removed D6 will ble D2 not visible [], [wen []5 Uneven D4 VIIILLI: Visible [].

Title:In Vitro Fertilization and Embryo Transfer
Author:Don P. Wolf
Publisher:Springer Science & Business Media - 2012-12-06


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