High-Performance Liquid Chromatography

High-Performance Liquid Chromatography

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High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.The problem of degassing (bubble formation) during a gradient run is particularly severe for reverse-phase LC, because ... This effect can be partially offset by running the gradient from 5 vol% organic/water to 95 vol% organic/water, rather than from 0 to 100% (19). 2. ... or drifting baseline during gradient elution, or to the appearance of artifactual peaks that do not come from the sample. ... 26b, for a reverse-phase gradient with a€œACS-gradea€ methanol as solvent B. Replacement ofanbsp;...

Title:High-Performance Liquid Chromatography
Author:Csaba Horváth
Publisher:Elsevier - 2013-10-22


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