Biochemical kinetics refers to the rate at which a reaction takes place. Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material. The Handbook of Biochemical Kinetics provides the qunderlying scaffoldingq of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on techniques and their likely limitations and pitfalls, as well as derivations of fundamental rate equations that characterize biochemical processes. Key Features * Over 750 pages devoted to theory and techniques for studying enzymic and metabolic processes * Over 1, 500 definitions of kinetic and mechanistic terminology, with key references * Practical advice on experimental design of kinetic experiments * Extended step-by-step methods for deriving rate equations * Over 1, 000 enzymes, complete with EC numbers, reactions catalyzed, and references to reviews and/or assay methods * Over 5, 000 selected references to kinetic methods appearing in the Methods in Enzymology series * 72-page Wordfinder that allows the reader to search by keywords * Summaries of mechanistic studies on key enzymes and protein systems * Over 250 diagrams, figures, tables, and structuresDiagram showing the addition of radiotracer A* at t 0 (termed the a#39;a#39;pulsea#39;a#39;) and the subsequent addition of excess unlabeled A (termed the a#39;a#39;chasea#39;a#39;). ... of high specific activity, 66, 529; preparation of folic acid specifically labeled with carbon- 13 in the benzoyl carbonyl, 66, 533; ... sources by affinity chromatography, 66, 709; preparation of an antiserum to sheep liver dihydropteridine reductase, 66, 723.
|Title||:||Handbook of Biochemical Kinetics|
|Author||:||Daniel L. Purich, R. Donald Allison|
|Publisher||:||Academic Press - 1999-10-26|