Guide to Yeast Genetics and Molecular Biology

Guide to Yeast Genetics and Molecular Biology

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Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study qYour Favorite Geneq in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and proteinThe population of polyadenylated products was selected based on a length threshold that excluded 95% of RNA in the ... reciprocal plot against the concentration of substrate, along with a linear fit (KM 1a„4 1500 nM, vmax 1a„4 180 pmol/min).

Title:Guide to Yeast Genetics and Molecular Biology
Publisher:Gulf Professional Publishing - 2004-05-14


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