These studies indicate that the E233G variant affects RAD51D protein interactions and functions and confers p53-dependent cisplatin resistance in breast cancer cell lines. This study is the first to analyze the functional effects of a clinically relevant RAD51D amino acid substitution. Since RAD51D functions in at least two possible mechanisms of chemoprotection, HR and telomere protection further study of this variant will provide insight into which cellular mechanism involving RAD51D functions in cellular response to anticancer agents.Then, the slides were stained with SYBR green (Trevigen) and comets scored using a Nikon epifluorescence ... For DSB repair studies, neutral comet assay was performed as described above after 0, 24 and 48 hours following the 3 hour anbsp;...
|Title||:||Functional Analysis of the Rad51d (E233G) Breast Cancer Associated Polymorphism and a Pharmacogenetic Evaluation of RAD51D Status|
|Publisher||:||ProQuest - 2008|