Understanding the dynamics of biopolymers in complex flows is critical for the successful design of lab-on-a-chip devices that hold promise in scaling down the size of traditional bench-top chemical and biological equipment to fit on a device the size of a typical credit card. This scaling yields many advantages including increased heat and mass transfer, reduction of contamination, and the possibility of massive serialization and parallelization that could greatly improve current analysis systems. However, successful design of these devices requires new knowledge of biomolecules' dynamics within these devices, as typical channel dimensions of these devices are on the same order of magnitude as the size of the molecules. Improper chip design might lead to shear degradation or cause irreversible damage to molecules, while successful designs could allow for the ability to control conformations resulting in increased reaction rates or separation efficiency. In the studies presented in this dissertation, we seek to characterize DNA dynamics and conformations in a model complex flow to gain insight into the rational design future lab-on-a-chip systems.Sink8 : Non-MOS clean wetsink with piranha and HF baths Spinnerl : A manual spin-coater used for deposting photoresists. ... Wet oxide growth: Tystar 4, recipe 4WET0XA, 1100AdC for 8 hrs 20 min + 1 hour ramp time + 1 hour cool time 2.
|Title||:||Controlling Dynamics and Conformations of DNA in Flow Through Microfabricated Post Arrays: A Single Molecule Study|
|Author||:||Nerayo Petros Teclemariam|
|Publisher||:||ProQuest - 2007|