Over the past ten years several sophisticated in vitro test systems based on epithelial cell cultures have been introduced in the field of drug delivery. These models have been found to be very useful in characterizing the permeability of drugs across epithelial tissues, and in studying formulations or carrier systems for improved drug delivery and enhanced absorption. Compared to in vivo trials on animals or humans, cell culture models are faster, more convenient and cost effective, ethically advantageous and, most importantly, they can be more easily standardized and validated. This book provides a practical approach to contemporary cell culture-based in vitro techniques for drug transport studies at biological absorption barriers. It is an invaluable source of information for students attending graduate courses on this subject and pharmaceutical scientists working in industry or in academia.333 21.1 Schematic diagram of a typical artificial neural network (ANN) with X- input nodes, eight hidden layer nodes, and one output node. 355 21.2 ... 373 22.1 Schematical figure of the principle of a confocal laser scanning microscope ( CLSM). ... 383 22.5 3D cross-sectioning through a microparticle prepared by complex coacervation after covalent labeling of gelatin with rhodamine-B- isothiocyanate.
|Title||:||Cell Culture Models of Biological Barriers|
|Publisher||:||CRC Press - 2003-09-02|