The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Key Features * Detailed, easy-to-follow, step-by-step protocols * Convenient, easy-to-use format * Extensive practical information * Essential background information * Helpful hintsPearson, W. R., and Lipman, D. J. (1988). ... aquot;Molecular cloningaA Laboratory Manual. ... repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: Construction of a heavy chain variable region-specific cDNA library. Proc.
|Author||:||Vedpal S. Malik, Erik P. Lillehoj|
|Publisher||:||Academic Press - 2013-10-22|